Other artificial insemination lab techniques

Reproductive medicine offers many different methods to help people have a child. We use many of them right here in our laboratory. The special lab techniques our clinic offers are now the standard in reproductive medicine.

Availability and use of these techniques during treatment greatly increases the chances of a successful cycle. From a scientific point of view, as we use each technique multiple times, we help move current medical knowledge and practice forward.

Your doctor and embryologist will advise you on the most suitable method for you based on your results.

Assisted hatching

Assisted hatching is a lab technique that uses micromanipulation (laser) to create a small hole in the embryo’s protective lining, called the zona pellucida. This makes it easier for the embryo to implant in the uterus.

Extended culture

Usually embryos are cultivated in the lab for three to five days. Embryos cultivated for five days are referred to as “extended cultures”, because on the fifth day embryos reach the blastocyst stage.  Transferring the embryo at this stage gives it a greater chance of successful implantation.

Cryopreservation

Cryopreservation is performed using a method called vitrification, which “flash freezes” at temperatures reaching -196 C°.  This is a quite gentle technique for freezing embryos and sperm, and the cell survival rate after thawing is very high (90%).

EmbryoGlue

EmbryoGlue is a special medium that increases the chances of successful conception. “Tissue adhesive” (hyaluronic acid) fixes embryos in place and helps them achieve better implantation. It also contains all the nutrients the embryo needs for optimal development after the transfer.

IVM

In vitro maturation (IVM) allows eggs to mature outside the body. This technique does not require hormonal stimulation of the ovaries like other methods. We recommend this technique for patients before beginning cancer treatments or where there is a risk of ovarian hyperstimulation syndrome. Eggs are retrieved from the ovaries before they are mature, and then spend 24 to 48 hours maturing in the lab. The mature eggs are then fertilized, cultivated and transferred into the uterus. This method is much gentler for some women, since unlike IVF the ovaries are subjected to little to no hormonal stimulation.

IMSI

Intracytoplasmic morphologically selected sperm injection (IMSI) is an assisted reproductive technique for collecting sperm using a special microscope. This microscope can provide much greater magnifying power than those normally used in reproductive laboratories (approx. 6000 times vs. 400 times). This increased magnification makes it easier to recognize and eliminate sperm displaying abnormalities with a negative impact on treatment.

ISME-T

Implantation support medium embryo transfer (ISME-T) increases the success rate for conception even before transferring embryos into the uterus. Patients come to the clinic three days after egg retrieval, and a small amount of the medium used for her embryo culture is transferred to her uterus using a thin catheter. The process is painless and gives embryos a better chance of successfully implanting once transferred to the uterus.

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